We are developing methods based mainly on Monte Carlo simulations to address specific problems in structural biology. We are focusing mainly on trying to understand structure-dynamics-function relationships in transmembrane proteins, particularly G-protein coupled receptors (GPCR). GPCR play a key role in the signaling network inside the cells, comprising about half of all the targets available for therapeutic drugs in medicine. GPCR regulate many physiological processes, including: neurotransmission, cell metabolism, cellular growth and differentiation, immune response, inflammatory processes, taste, smell and vision. One particular class of GPCR we have been interested in are serotonin receptors, relevant for understanding the behavioral effects of hallucinogens and for the treatment of hallucinogen-based syndromes and drug abuse.

A fundamental problem with these systems is that there is little or no structural information available. Without knowledge of their structure there is no hope to rationalize their biological function, let alone to design or improve therapeutic drugs.

Therefore, most of our efforts during the last years have focused in trying to design protocols to bringing some structural insight into these very important proteins. However, the design of searching techniques alone is insufficient to achieve success, and must be accompanied by continual improvements of the energy function used in the computational search. This, in turn, requires insight into the physics of the interactions, as discussed above.
 



S A Hassan, PhD (main page)